The University Main Library also known as Prempeh II Library is the management centre for all library operations within the University. It provides staff and technical services as well as coordinates the activities of the College Libraries. The University Librarian is the administrative and technical head of the University Library System, which is made up of the Prempeh II Library and the six college libraries. He is directly responsible to the Vice-Chancellor in all administrative, technical and professional matters concerning the libraries in the University.

The University Librarian is supported by two Deputy Librarians and five departmental heads in the effective and efficient running of the library. These heads of department are the means through which power and authority from the University Librarian flows down to the para-professionals and the Junior Library Assistants.

The Prempeh II Library has five functional departments; Administration, Collection Development and Management, Students Support, Academic Support, Library Systems  & Support.

On the ground floor of the Main Library are the Educational Zone, Lending Department (which houses the Issue Desk and the Catalogues), the Discussion Area, Photocopying Unit, Collection and Development Management and the Systems and Support Departments respectively.

The first floor contains offices and  Reading area. The busts of former Vice-Chancellors of the University as well as ingenious African Americans are displayed on the same floor. The adjoining room is a Lounge for group discussion.

The Ghana Collection are on the second floor of the Old block. The adjoining room accommodates the Reading Area. On the second floor of the new block are the Science and Technology Collections of the Students’ Reference Library.

The third floor of the old block has the Seminar room. The Health and Allied Science Library Collections is in the Third Floor of the new block.

On the fourth floor of the extension are the collections on Humanities and Social Science.